Coding

Part:BBa_K2381003:Design

Designed by: Zhujun Xia   Group: iGEM17_HZAU-China   (2017-10-20)


cod1 + pMag

A fusion protein of pMagFast and Cod1, which linked by GS linker.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to prevent the fusion protein has steric hindrance, We use a 12aa GS linker to link the light-induced dimerization protein with a part of dCas9, which split by a conventional strategy. According to the paper, this conventional strategy is effective by rapamycin induced.

Source

The light-induced protein pMagFast is made from the VVD protein family derived from the fungus Neurospora crassa. The cod1 is made from the dCas9 Protein derived from the Streptococcus pyogenes.

References

Nihongaki, Y., et al. (2015). "Photoactivatable CRISPR-Cas9 for optogenetic genome editing." Nat Biotechnol 33(7): 755-760.